| 2018-25-15 | Wings | Jan 22, 2019 | Airframe (727-227) |
| 2017-19-02 | Doors | Oct 19, 2017 | Airframe (727-227)Only with: Equipped with a main cargo door (MCD). |
| 2014-04-09 | Wings | May 2, 2014 | Airframe (727-227) |
| 2013-24-15 | Fuel | Jan 8, 2014 | Airframe (727-227) |
| 2013-21-07 | Stabilizers | Dec 5, 2013 | Airframe (727-227)Serial numbers per Boeing Service Bulletin 55-46, dated April 8, 1970 (Group III and Group IV). Only with: Airplanes identified as Group III and Group IV in Boeing Service Bulletin 55-46. |
| 2013-22-03 | Fuel | Dec 5, 2013 | Airframe (727-227) |
| 2013-19-08 | Stall Warning System | Nov 6, 2013 | Airframe (727-227)Serial numbers per Boeing Special Attention Service Bulletins 727-34-0245, 737-34-2102, and 747-34-2925 |
| 2013-16-17 | Wings | Sep 27, 2013 | Airframe (727-227) |
| 2013-15-11 | Stabilizers | Sep 20, 2013 | Airframe (727-227) |
| 2013-15-15 | Fuselage | Sep 20, 2013 | Airframe (727-227)Serial numbers per Boeing Special Attention Service Bulletin 727-53-0234, dated January 17, 2013 |
| 2013-14-02 | Wings | Aug 26, 2013 | Airframe (727-227) |
| 2013-14-03 | Stabilizers | Aug 26, 2013 | Airframe (727-227) |
| 2011-12-05 | The Boeing Company Model 727, 727C, 727-100, 727-100C, 727-200, and 727-200F Series… | Jul 15, 2011 | Airframe (727-227) |
| 2011-01-05 | The Boeing Company Model 727, 727C, 727-100, 727-100C, 727-200, and 727-200F Series… | Feb 14, 2011 | Airframe (727-227) |
| 2008-04-10 R1 | The Boeing Company Model 727 Airplanes | Dec 30, 2009 | Airframe (727-227) |
| 2009-20-08 | Boeing Model 727 Airplanes | Nov 5, 2009 | Airframe (727-227) |
| 2009-20-03 | Boeing Model 727 Airplanes | Nov 3, 2009 | Airframe (727-227) |
| 2008-22-12 | Boeing Model 727 Airplanes | Dec 17, 2008 | Airframe (727-227) |
| 2008-11-09 | Boeing Model 727 Airplanes | Jul 3, 2008 | Airframe (727-227) |
| 2008-02-13 | Boeing Model 727 Airplanes | Feb 28, 2008 | Airframe (727-227) |
| 2007-21-04 | Boeing Model 727 Airplanes | Nov 16, 2007 | Airframe (727-227) |
| 2007-12-14 | Boeing Model 727 Airplanes | Jul 16, 2007 | Airframe (727-227) |
| 2006-13-16 | Boeing Model 727 Airplanes | Aug 1, 2006 | Airframe (727-227)Serial numbers per Boeing Special Attention Service Bulletin 727-27-0234, dated November 10, 2005 |
| 2006-09-03 | Boeing Model 727, 727C, 727-100, and 727-100C Series Airplanes | May 31, 2006 | Airframe (727-227) |
| 2005-23-07 | Boeing Model 727 Airplanes | Dec 13, 2005 | Airframe (727-227) |
| 2005-20-33 | Boeing Model 727, 727C, 727-100, and 727-100C Series Airplanes | Nov 16, 2005 | Airframe (727-227)Certain serial numbers: 1–694. Only with: Line numbers 1 through 694 inclusive. |
| 2005-20-13 | Boeing Model 727 Airplanes | Nov 9, 2005 | Airframe (727-227) |
| 2005-19-24 | Boeing Model 727 Series Airplanes | Oct 27, 2005 | Airframe (727-227) |
| 2005-16-07 | Boeing Model 727 Airplanes | Sep 13, 2005 | Airframe (727-227) |
| 2005-14-07 | Boeing Model 727, 727C, 727-100, 727-100C, 727-200, and 727-200F Series Airplanes | Aug 15, 2005 | Airframe (727-227)Serial numbers per Boeing Alert Service Bulletin 727-57A0135, Revision 3, dated June 27, 2002 |
| 2005-13-40 | Boeing Model 727 Airplanes, Equipped With an Auxiliary Fuel Tank Having a Fuel Pump… | Aug 4, 2005 | Airframe (727-227)Only with: Equipped with an auxiliary fuel tank having a fuel pump installed. |
| 2004-09-36 | Boeing Model 727 Series Airplanes | Jun 15, 2004 | Airframe (727-227) |
| 2004-03-30 | Boeing Model 727, 727C, 727-100, and 727-100C Series Airplanes | Mar 24, 2004 | Airframe (727-227)Serial numbers per Boeing Alert Service Bulletin 727-53A0225, dated September 11, 2003 |
| 2004-03-31 | Boeing Model 727, 727-100C, 727-200F, and 727C Series Airplanes | Mar 24, 2004 | Airframe (727-227)Serial numbers per Boeing Alert Service Bulletin 727-53A0226, dated September 11, 2003 |
| 2003-03-15 R1 | Various Boeing and McDonnell Douglas Transport Category Airplanes | Dec 22, 2003 | Airframe (727-227) |
| 2003-11-03 | Boeing Model 727, 737-100, 737-200, and 737-200C Series Airplanes | Jul 3, 2003 | Airframe (727-227)Certain serial numbers: 727 L/N 1–L/N 1832; 737-100 L/N 1–L/N 1033; 737-200 L/N 1–L/N 1033; 737-200C L/N 1–L/N 1033. Only with: Applicability limited by line numbers rather than serial numbers. |
| 2003-03-15 | Various Boeing and McDonnell Douglas Transport Category Airplanes | Mar 7, 2003 | Airframe (727-227) |
| 2002-24-05 | Boeing Model 727 Series Airplanes | Dec 18, 2002 | Airframe (727-227)Certain serial numbers: 1–1832 |
| 2002-17-05 | Boeing Model 727 Series Airplanes | Sep 16, 2002 | Airframe (727-227) |
| 2002-16-19 | Boeing Model 727 Series Airplanes Modified in Accordance With Supplemental Type… | Sep 19, 2002 | Airframe (727-227)Only with: Converted from passenger to cargo-carrying (freighter) configuration in accordance with STC SA1767SO or SA1768SO. |
| 2002-16-20 | Boeing Model 727 Series Airplanes Modified in Accordance With Supplemental Type… | Sep 19, 2002 | Airframe (727-227)Only with: Airplanes converted from passenger to cargo (freighter) configuration in accordance with STC SA1368SO, SA1797SO, or SA1798SO. |
| 2002-16-21 | Boeing Model 727 Series Airplanes Modified in Accordance with Supplemental Type… | Sep 19, 2002 | Airframe (727-227)Only with: Converted from passenger to cargo-carrying (freighter) configuration in accordance with STC ST00015AT. |
| 2002-16-22 | Boeing Model 727 Series Airplanes Modified in Accordance With Supplemental Type… | Sep 19, 2002 | Airframe (727-227)Only with: Converted from passenger to cargo-carrying (freighter) configuration in accordance with STC SA1444SO, SA1509SO, SA1543SO, or SA1896SO. |
| 2002-12-13 | Boeing Model 727 Series Airplanes | Jul 31, 2002 | Airframe (727-227) |
| 2001-22-12 | Boeing Model 727 Series Airplanes | Nov 15, 2001 | Airframe (727-227) |
| 2001-20-09 | Boeing Model 727 Series Airplanes | Nov 15, 2001 | Airframe (727-227) |
| 2000-14-07 | Boeing Model 727 Series Airplanes | Aug 17, 2000 | Airframe (727-227)Certain serial numbers: 727 858–864; 727 867–869; 727 872–883; 727 885–1832. Only with: Limited by line numbers rather than serial numbers. |
| 90-15-12 R1 | Boeing Model 727 Series Airplanes Modified in Accordance with Valsan Supplemental Type… | Aug 15, 2000 | Airframe (727-227)Only with: Modified by installation of Pratt and Whitney JT8D-217C or -219 engines in accordance with Valsan STC SA4363NM. |
| 2000-02-19 | Boeing Model 727 Series Airplanes | Mar 9, 2000 | Airframe (727-227)Serial numbers per Boeing Service Bulletin 727-57-0177, dated December 22, 1988 |
| 99-20-10 | Boeing Model 727 Series Airplanes | Nov 8, 1999 | Airframe (727-227)Certain serial numbers: 124; 126; 130; 146; 221; 287; …. Only with: Applies to airplanes with the listed line numbers (not serial numbers). |
| 99-04-22 | Boeing Model 727, 727-100, 727-200, 727C, 727-100C, and 727-200F Series Airplanes | Mar 4, 1999 | Airframe (727-227) |
| 98-26-18 | Boeing Model 727 Series Airplanes Modified in Accordance With Supplemental Type… | Feb 16, 1999 | Airframe (727-227)Only with: Converted from passenger to cargo-carrying (freighter) configuration in accordance with STC SA1767SO, SA1768SO, or SA7447SW. |
| 98-26-19 | Boeing Model 727 Series Airplanes Modified in Accordance With Supplemental Type… | Feb 16, 1999 | Airframe (727-227)Only with: Converted from passenger to cargo-carrying (freighter) configuration in accordance with STC SA1368SO, SA1797SO, or SA1798SO. |
| 98-26-20 | Boeing Model 727 Series Airplanes Modified in Accordance With Supplemental Type… | Feb 16, 1999 | Airframe (727-227)Only with: Converted from passenger to cargo-carrying (freighter) configuration in accordance with STC ST00015AT. |
| 98-26-21 | Boeing Model 727 Series Airplanes Modified in Accordance With Supplemental Type… | Feb 16, 1999 | Airframe (727-227)Only with: Converted from passenger to cargo-carrying (freighter) configuration in accordance with STC SA1444SO, SA1509SO, SA1543SO, SA1896SO, SA1740SO, or SA1667SO. |
| 98-11-03 R1 | Boeing Model 727 Series Airplanes | Jun 23, 1998 | Airframe (727-227) |
| 98-23-51 | Boeing Model 727 Series Airplanes | Dec 14, 1998 | Airframe (727-227)Certain serial numbers: 727 1–849. Only with: Line positions 1 through 849 inclusive that have been converted from passenger to cargo (freighter) or passenger-and-cargo (combi) configuration. |
| 98-18-20 | Boeing Model 727 and Model 737 Series Airplanes Equipped with J.C. Carter Company Fuel… | Oct 21, 1998 | Airframe (727-227)Only with: Equipped with J.C. Carter Company fuel valve actuators P/N 40574-2 (Kearfott Models 3715-7 and -8) or 40574-5 (Kearfott Model 3715-9). |
| 98-11-03 | Boeing Model 727 Series Airplanes | Jun 23, 1998 | Airframe (727-227) |